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|aNTTU|bG|cE047555|d572.64|eP967|pBOOK|fCKY|zBOOK|m0|tDDC
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|aProteomics in practice :|ba guide to successful experimental design /|cedited by Reiner Westermeier, Tom Naven, Hans-Rudolf Hopker.
|
250 |
|
|a2nd ed.
|
260 |
|
|aWeinheim :|bWiley-VCH ;|aChichester :|bJohn Wiley [distributor],|c2008.
|
300 |
|
|axx, 482 p. :|bill. (some col.) ;|c25 cm.
|
440 |
0
|
|a2009年教育部專案充實圖儀設備購書
|
500 |
|
|aPrevious ed.: 2002.
|
504 |
|
|aIncludes bibliographical references and index.
|
505 |
0
|
|aPreface -- Foreword -- Abbreviations, symbols, units -- Introduction -- Pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions -- Pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search -- Pt. III. Trouble shooting -- Glossary of terms.
|
650 |
0
|
|aProteins|xAnalysis.
|
650 |
0
|
|aMolecular biology.
|
650 |
12
|
|aProteomics.
|
650 |
22
|
|aProteins|xanalysis.
|
650 |
22
|
|aMolecular Biology.
|
700 |
1
|
|aWestermeier, Reiner.
|
700 |
1
|
|aNaven, Tom.
|
700 |
1
|
|aHopker, Hans-Rudolf.
|
809 |
|
|d572.64|eP967|tDDC|pBOOK
|